Inv A gene specific PCR for detection of Salmonella from broilers
نویسندگان
چکیده
منابع مشابه
Detection of InvA Gene in Isolated Salmonella from Broilers by PCR Method
The presence of Salmonella was detected in 192 samples of poultry carcasses from poultry farms in Shiraz province (Iran). A total of 30 Salmonella isolates were found in chicken samples (15.6%), by conventional culturing and confirmed by PCR and serology methods. Strains of serogroup D1 were the most prevalent strains, followed by serogroups C1, B and C2. All strains were subjected to Salmonell...
متن کاملPCR amplification of the fimA gene sequence of Salmonella typhimurium, a specific method for detection of Salmonella spp.
The goal of this study was to evaluate the suitability of the fimA gene amplification by PCR as a specific method for detection of Salmonella strains. Salmonella typhimurium and other pathogenic members of the family Enterobacteriaceae produce morphologically and antigenically related, thin, aggregative, type 1 fimbriae. A single gene, fimA, encodes the major fimbrial unit. In order to obtain h...
متن کاملrapid and specific detection of salmonella typhimurium by pcr-elisa
objectives: salmonella typhimurium is important food-borne pathogen responsible for gastroenteritis. in this work a polymerase chain reaction based enzyme linked immunosorbent assay (pcr-elisa) was developed to identify salmonella typhimurium. materials and methods: the rfb gene which is responsible for biosynthesis of the salmonella o-antigenic lipopolysaccharide was selected as the target s...
متن کاملa rapid and specific pcr–elisa for detecting salmonella typhi
background salmonella continues to be a major food borne pathogen for animals and humans. a sensitive and specific pcr–elisa technique was developed to detect salmonella typhi. materials and methods the assay was based on the incorporation of digoxigenin-labeled dutp and a biotin-labeled primers specific for rfbe gene during pcr amplification. the labeled pcr products were bound to streptoavidi...
متن کاملThe detection of Klebsiella pneumoniae 16srRNA specific gene by PCR-ELISA technique
Objective: Klebsiella Pneumoniae is one of the most important infection bacteria of the Enterobacteriaceae family. It is also the second factor of hospital bacteremia due to the gram negative bacteria after Escherichia coli. In this research, this bacteria with the private 16SrDNA gene, was identified through PCR-ELISA technique. Materials and Methods: In this method the dNTP labeled with Digox...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Veterinary World
سال: 2011
ISSN: 0972-8988
DOI: 10.5455/vetworld.2011.562-564